Phospho-ERK1-T202/Y204 + ERK2-T185/Y187 Rabbit Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using Phospho-ERK1-T202/Y204 + ERK2- T185/Y187 Rabbit mAb at 1:1000 dilution.Both 293T cells and NIH/3T3 cells were treated by ATP(5 mM) at 30℃ for 1 hour. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus. Exposure time: 10s.
| Catalog Number: | MAB-94754 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | ARC1595 |
| Immunogen: | A synthetic phosphorylated peptide around T202 & Y204 of human ERK1MK03 ( |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 |
| Molecular Weight: | 42kDa/44kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | ERK-1; ERK1; ERT2; HS44KDAP; HUMKER1A; P44ERK1; P44MAPK; PRKM3; p44-ERK1; p44- MAPK |
Applications
Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Immunogen
A synthetic phosphorylated peptide around T202 & Y204 of human ERK1MK03 (