Phospho Histone H2A.X (Ser139) Rabbit Polyclonal Antibody
Western blot analysis of lysates from 1) 4T1, 2) 293 cells, (Green) primary antibody was diluted at 1:1000, 4°over night, Dylight 800 secondary antibody was diluted at 1:10000, 37° 1hour. (Red) Actin β Monoclonal Antibody(5B7)
| Catalog Number: | ABP-0640 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human Histone H2A.X around the phosphorylation site of Ser139. AA range:94-143. |
| Reactivity: | Human;Mouse;Rat;Hamster |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 Immunofluorescence: 1:50-200 ELISA: 1:10000 |
| Molecular Weight: | 15 kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | Liquid |
| Synonyms: | H2AFX; H2AX; Histone H2A.x; H2a/x |
Applications
Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:300 Immunofluorescence: 1:50-200 ELISA: 1:10000
Immunogen
The antiserum was produced against synthesized peptide derived from human Histone H2A.X around the phosphorylation site of Ser139. AA range:94-143.