Phospho-Histone H2AFX-S139
Western blot analysis of extracts of various cell lines, using Phospho-Histone H2AX-S139 antibody at 1:1000 dilution. Jurkat cells were treated by Etoposide (25 uM) for 5 hours. NIH/3T3 cells were treated by UV for 15-30 minutes. C6 cells were treated by UV for 15-30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus Exposure time: 5s.
| Catalog Number: | MAB-94464 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | A09 |
| Immunogen: | A synthetic phosphorylated peptide around S139 of human Histone H2AX |
| Reactivity: | Hu |
| Applications: | Western Blot: 1:1000 Immunohistochemistry: 1:50 Immunofluorescence: 1:50 |
| Molecular Weight: | 17kDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | H2AFX; H2A.X; H2A/X; H2AX; histone H2AX |
Applications
Western Blot: 1:1000 Immunohistochemistry: 1:50 Immunofluorescence: 1:50
Immunogen
A synthetic phosphorylated peptide around S139 of human Histone H2AX