Phospho-Met (Y1349)

Western blot analysis of extracts of HeLa cells, using Phospho-MET-Y1349 antibody at 1:1000 dilution. HeLa cells were treated by HGF (40ng/ml) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Western blot analysis of extracts of HeLa cells, using Phospho-MET-Y1349 antibody at 1:1000 dilution. HeLa cells were treated by HGF (40ng/ml) for 10 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit. Exposure time: 90s.

Catalog Number:ABP11206
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A phospho specific peptide corresponding to residues surrounding Y1349 of human MET
Reactivity:Hu
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:155.541 kDa
Purification:Affinity purification
Form:liquid

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

A phospho specific peptide corresponding to residues surrounding Y1349 of human MET