Phospho-MYC-T58
Western blot analysis of extracts of 293T cells, using Phospho-MYC-T58 antibody at 1:500 dilution.293T cells were treated by Calyculin A (100 nM) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s
| Catalog Number: | ABP-0080 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | Poly |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding T58 of human MYC. |
| Reactivity: | Hu |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100 |
| Molecular Weight: | 62KDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | MRTL;MYCC;bHLHe39;c-Myc;MYC |
Applications
Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100
Immunogen
A phospho specific peptide corresponding to residues surrounding T58 of human MYC.