Phospho--p70 S6k (S235/236)
Western blot analysis of extracts of various cell lines, using Phospho-S6 Ribosomal Protein (RPS6)-S235/236 antibody at 1:1000 dilution.HeLa cells NIH/3T3 cells and PC-12 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight.NIH/3T3 cells were treated by PDGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.
| Catalog Number: | ABP-0538 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | Poly |
| Immunogen: | A synthetic phosphorylated peptide around S235 & S236 of human RPS6. |
| Reactivity: | Hu |
| Applications: | Western Blot: 1:500 - 1:2000 |
| Molecular Weight: | 32KDa |
| Form: | liquid |
| Synonyms: | RPS6;S6;RPS6 |
Applications
Western Blot: 1:500 - 1:2000
Immunogen
A synthetic phosphorylated peptide around S235 & S236 of human RPS6.