Phospho-p70 S6k (S371) Rabbit Polyclonal Antibody
Western blot analysis of NIH/3T3, using Phospho-p70 S6K (S371) antibody at 1:400 dilution. NIH/3T3 cells were treated by EGF (100 ng/ml) at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
| Catalog Number: | ABP-10854 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | Poly |
| Immunogen: | A synthetic phosphorylated peptide around S371 of human P70 S6K |
| Reactivity: | Hu |
| Applications: | Western Blot: 1:500 - 1:2000 |
| Molecular Weight: | 70kDa |
| Purification: | Affinity purification |
| Form: | liquid |
| Synonyms: | PS6K;S6K;S6K-beta-1;S6K1;STK14A;p70 S6KA;p70(S6K)-alpha;p70-S6K;p70-alpha;P70 S6K;RPS6KB1;p70S6KA |
Applications
Western Blot: 1:500 - 1:2000
Immunogen
A synthetic phosphorylated peptide around S371 of human P70 S6K