Immunological Sciences

Phospho-P70 S6K1 (T389)

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-P70S6K1-T389 at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plusa Exposure time: 10s.

Western blot analysis of extracts of NIH/3T3 cells, using Phospho-P70S6K1-T389 at 1:1000 dilution.NIH/3T3 cells were treated by 10% FBS at 37℃ for 30 minutes after serum-starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plusa Exposure time: 10s.

Catalog Number:AB-83589
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Reactivity:Hu
Applications:WB: 1:1000
Molecular Weight:70 kDa
Purification:Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues around Thr389 of human p70 S6 kinase. Antibodies are purified by protein A and peptide affinity chromatography.
Form:liquid

Applications

WB: 1:1000