Phospho-Smad5-S463/465
Western blot analysis of extracts of various cells, using Phospho-Smad5- S463/465 antibody. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
| Catalog Number: | ABP-0629 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | Poly |
| Immunogen: | A phospho specific peptide corresponding to residues surrounding S463/465 of human Smad5 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:1000 Immunohistochemistry(paraffin-embedded tissues): 1:50-1:100 Immunofluorescence: 1:50-1:100 |
| Molecular Weight: | 52 kDa |
| Purification: | Affinity purification |
| Form: | liquid |
Applications
Western Blot: 1:500-1:1000 Immunohistochemistry(paraffin-embedded tissues): 1:50-1:100 Immunofluorescence: 1:50-1:100
Immunogen
A phospho specific peptide corresponding to residues surrounding S463/465 of human Smad5