Phospho-Stat1 (Tyr701) Rabbit Polyclonal Antibody
Western blot analysis of lysates from 1) MOUSE-BRAIN, 2) SY-SY5X ,3) COS7 cells, （Green）primary antibody was diluted at 1:1000, 4°over night, secondary antibody(cat:RS23920)was diluted at 1:10000, 37° 1hour. （Red） Actin β Monoclonal Antibody(5B7) antibody was diluted at 1:5000 as loading control, 4° over night,secondary antibody was diluted at 1:10000, 37° 1hour.
| Catalog Number: | ABP-0135 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | Poly |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human STAT1 around the phosphorylation site of Tyr701. AA range:668-717. |
| Reactivity: | Human;Mouse;Rat;Monkey |
| Applications: | Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300 Immunoprecipitation: 2-5 ug/mg lysate ELISA:1/10000 |
| Molecular Weight: | 87 kDa |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | liquid |
| Synonyms: | STAT1; Signal transducer and activator of transcription 1-alpha/beta; Transcription factor ISGF-3 components p91/p84 |
Applications
Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300 Immunoprecipitation: 2-5 ug/mg lysate ELISA:1/10000
Immunogen
The antiserum was produced against synthesized peptide derived from human STAT1 around the phosphorylation site of Tyr701. AA range:668-717.