Immunological Sciences

Phospho-Stat3 (S727)

Western blot analysis of extracts from serum-starved U266 cells, untreated (-) or treated with Human Interferon-1 (hIFN-1; 50ng/ml, 15 min; +), Hela cells, untreated (-) or treated with Human Tumor Necrosis Factor- (hTNF-; 20ng/ml, 30 min; +), HT-29 cells growing asynchronously (-) or arrested in mitosis by treatment with Thymidine (Thy; 2mM, 17 hours) followed by Nocodazole (Noc; 100ng/ml, 24 hrs; +), and NIH/3T3 cells, untreated (-) or treated with Human Platelet Derived Growth Factor- (hPDGF-AA; 50ng/ml, 30min; +) using Phospho-Stat3 (Ser727) Rabbit pAb (upper) or total Stat3 (124H6) Mouse (lower).

Western blot analysis of extracts from serum-starved U266 cells, untreated (-) or treated with Human Interferon-1 (hIFN-1; 50ng/ml, 15 min; +), Hela cells, untreated (-) or treated with Human Tumor Necrosis Factor- (hTNF-; 20ng/ml, 30 min; +), HT-29 cells growing asynchronously (-) or arrested in mitosis by treatment with Thymidine (Thy; 2mM, 17 hours) followed by Nocodazole (Noc; 100ng/ml, 24 hrs; +), and NIH/3T3 cells, untreated (-) or treated with Human Platelet Derived Growth Factor- (hPDGF-AA; 50ng/ml, 30min; +) using Phospho-Stat3 (Ser727) Rabbit pAb (upper) or total Stat3 (124H6) Mouse (lower).

Catalog Number:ABP-4002
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Reactivity:Hu, Ms, Rt, Monkey
Applications:Western Blotting 1:1000 Immunohistochesmistry 1:100-1:300 Immunoprecipitation: 1:200-500 ELISA: 1:10000
Molecular Weight:86 kDa
Purification:Polyclonal antibody is produced by immunizing animals with a synthetic phospho-peptide corresponding to residues surrounding Ser727 of human Stat3 protein.
Form:liquid

Applications

Western Blotting 1:1000 Immunohistochesmistry 1:100-1:300 Immunoprecipitation: 1:200-500 ELISA: 1:10000