Phospho-STAT5A-Y694

Western blot analysis of extracts ofK562 cells, using Phospho-STAT5A-Y694 antibody  at 1:2000dilution. K562 cells were treated byG-CSF (25ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA. Detection: ECL Exposure time: 90s

Western blot analysis of extracts ofK562 cells, using Phospho-STAT5A-Y694 antibody at 1:2000dilution. K562 cells were treated byG-CSF (25ng/ml) for 30 minutes after serum-starvation overnight.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA. Detection: ECL Exposure time: 90s

Catalog Number:ABP-0138
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:Poly
Immunogen:A phospho specific peptide corresponding to residues surrounding Tyr694 of human STAT5A
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:1000
Molecular Weight:105kDa
Purification:Affinity purification
Form:liquid

Applications

Western Blot: 1:1000

Immunogen

A phospho specific peptide corresponding to residues surrounding Tyr694 of human STAT5A