Phospho-YAP1-S127 pAb
Western blot analysis of extracts of NIH3T3 cells, using Phospho-YAP1-S127 antibody at 1:1000 dilution.NIH3T3 cells were treated by Sorbitol (0.2M) at 37℃ for 60 minutes after serum starvation overnight. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL West Pico Plus Exposure time: 60s.
| Catalog Number: | ABP-0489 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic phosphorylated peptide around S127 of human YAP1. |
| Reactivity: | Hu, Ms,Rt |
| Applications: | Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100 |
| Molecular Weight: | 68kDa |
| Form: | liquid |
| Synonyms: | COB1;YAP;YAP2;YAP65;YKI;YAP1 |
Applications
Western Blot: 1:500 - 1:1000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:100
Immunogen
A synthetic phosphorylated peptide around S127 of human YAP1.