PLP1 Rabbit Polyclonal Antibody

Western blot analysis of various lysates, using PLP1 Rabbit pAb  at 1:2000dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 30s.

Western blot analysis of various lysates, using PLP1 Rabbit pAb  at 1:2000dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.

Catalog Number:AB-83554
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 90-150 of human PLP1.
Reactivity:Human,Mouse,Rat
Applications:Western Blot:   1:1000 - 1:5000 Immunohistochemistry (paraffin-embedded tissues):  1:50 - 1:200 Immunofluorescence:  1:50 - 1:200 Immunocytochemistry:   1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL.
Molecular Weight:20-25kDa
Purification:Affinity purification
Form:Liquid
Synonyms:PLP; PMD; HLD1; MMPL; SPG2; GPM6C; PLP/DM20; PLP1

Applications

Western Blot:   1:1000 - 1:5000 Immunohistochemistry (paraffin-embedded tissues):  1:50 - 1:200 Immunofluorescence:  1:50 - 1:200 Immunocytochemistry:   1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL.

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 90-150 of human PLP1.