PRDM1

Western blot analysis of PRDM1/Blimp1 using anti- PRDM1/Blimp1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: rat spleen tissue lysates, Lane 3: rat stomach tissue lysates, Lane 4: rat lung tissue lysates, Lane 5: mouse thymus tissue lysates, Lane 6: mouse spleen tissue lysates, Lane 7: mouse stomach tissue lysates, Lane 8: mouse lung tissue lysates, Lane 9: mouse HEPA1-6 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PRDM1/Blimp1 antigen affinity purified polyclonal antibody at 0.5 μg/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PRDM1/Blimp1 at approximately 92KD. The expected band size for PRDM1/Blimp1 is at 92KD.