Rabbit anti mCherry-Tag Monoclonal Antibody
Western blot analysis of lysates from wild type (WT) and 293F cells transfected with CT55-mCherry-Tag using mCherry-Tag Rabbit mAb at 1:6800 dilution incubated overnight at 4℃. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 30 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. .Exposure time: 20s.
| Catalog Number: | MAB-94804 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | 125C |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of Discosoma MCherry fluorescent proteinm. |
| Reactivity: | Species independent |
| Applications: | Western Blot: 1:2000 - 1:10000 Immunoprecipitation:0.5μg-4μg antibody for 200μg-600μg extracts of whole cellss |
| Molecular Weight: | 55kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | mCherry; mCherry tag; mCherry-tag |
Applications
Western Blot: 1:2000 - 1:10000 Immunoprecipitation:0.5μg-4μg antibody for 200μg-600μg extracts of whole cellss
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-236 of Discosoma MCherry fluorescent proteinm.