SIRT1
Western blot analysis of extracts from normal (control) and SIRT1 knockout HeLa cells, using SIRT1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 10s.
| Catalog Number: | AB-82681 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100 |
| Molecular Weight: | 130kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | SIR2;SIR2L1;SIR2alpha;SIRT1;sirtuin 1 |
Applications
Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:100
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 448-747 of human SIRT1.