SLC7A11 / xCT Rabbit Polyclonal Antibody

Western blot analysis of lysates from wild type (WT) and SLC7A11/xCT knockdown (KD) HeLa cells using [KD Validated] SLC7A11/xCT Rabbit pAb at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 60s.

Western blot analysis of lysates from wild type (WT) and SLC7A11/xCT knockdown (KD) HeLa cells using [KD Validated] SLC7A11/xCT Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:AB-84812
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SLC7A11/xCT.
Reactivity:Human,Mouse,Rat
Applications:Western Blot:     1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay  requirements.
Molecular Weight:42kDa
Purification:Affinity purification
Form:Liquid
Synonyms:xCT; CCBR1; SLC7A11/xCT

Applications

Western Blot:     1:500 - 1:1000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay  requirements.

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 201-300 of human SLC7A11/xCT.