Sonic Hedgegog (SHH) Rabbit Monoclonal Antibody

Western blot analysis of various lysates using Sonic Hedgehog (Shh) Rabbit mAb at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L)  at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.   
Exposure time: 60s.

Western blot analysis of various lysates using Sonic Hedgehog (Shh) Rabbit mAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.

Catalog Number:MAB-94796
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:ARC0701
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Sonic Hedgehog (Shh)
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:50kDa/27kDa
Purification:Affinity purification
Form:Liquid
Synonyms:TPT; HHG1; HLP3; HPE3; SMMCI; ShhNC; TPTPS; MCOPCB5; Sonic Hedgehog (Shh) Product Information

Applications

Western Blot: 1:500 - 1:2000 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 200-300 of human Sonic Hedgehog (Shh)