SPP1

Western blot analysis of extracts of
various cell lines, using Osteopontin
antibody at 1:500 dilution.
Secondary antibody: HRP Goat Anti-
Rabbit IgG (H+L) at 1:10000
dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in
TBST.
Detection: ECL West Pico Plus.  
Exposure time: 3min.

Western blot analysis of extracts of various cell lines, using Osteopontin antibody at 1:500 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Catalog Number:AB-84206
Size:100ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human SPP1.
Reactivity:Hu, Ms
Applications:Western Blot: 1:500 - 1:2000
Molecular Weight:35kDa/6kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:BNSP;BSPI;ETA-1;OPN;Osteopontin;SPP1

Applications

Western Blot: 1:500 - 1:2000

Immunogen

Recombinant fusion protein containing a sequence corresponding to amino acids 17-314 of human SPP1.