SQSTM1/ P62
Western blot analysis of extracts of various cell lines, using 62/SQSTM1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Kit. Exposure time: 10s.
| Catalog Number: | AB-83779 |
| Conjugate: | Unconjugated |
| Size: | 100ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human P62/SQSTM1 |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200 |
| Molecular Weight: | 62kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
Applications
Western Blot: 1:500-1:2000 Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-440 of human P62/SQSTM1