SUMO 2/3 Rabbit Polyclonal Antibody

Western blot analysis of extracts of various cell lines, using SUMO2/3 Rabbit pAb at 1:500
dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL West Pico Plus.
Exposure time: 3min.

Western blot analysis of extracts of various cell lines, using SUMO2/3 Rabbit pAb at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 3min.

Catalog Number:AB-84757
Conjugate:Unconjugated
Size:100 ug
Concentration:1mg/ml
Host:Rabbit
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 1-95 of human SUMO2/3.
Reactivity:Human, Mouse, Rat
Applications:Western Blot: 1:100 - 1:500 Immunohistochemistry (paraffin embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200
Molecular Weight:17kDa
Purification:Affinity purification
Form:Liquid
Synonyms:HSMT3; SMT3B; SUMO3; Smt3A; SMT3H2

Applications

Western Blot: 1:100 - 1:500 Immunohistochemistry (paraffin embedded tissues): 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-95 of human SUMO2/3.