td-Tomato

Western blot analysis of HEK293 cell lysates, and recombinantprotein solutions using rabbit pAb to td-Tomato, td-Tomato,dilution 1:1,000, in green [1] protein standard, [2] HEK293, [3]HEK293 cells transfected with td-Tomato-HA construct, [4] td-Tomato recombinant protein, [5] GFP recombinant protein, and [6] EK293transfected with a full length GFP construct. A major band at about28kDa corresponds to td-Tomato protein. The td-Tomato antibody does not react with GFP protein. The same blot was simultaneously probed with chicken pAb to HSP60, dilution 1:5,000 in red which reveals a band at 60kDa only in cell lysates.