TGS101

Western blot analysis of TSG101 expression in Jurkat cell lysate Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. 50ug of sample was loaded each laneunder reducing conditions. After Electrophoresis, proteins were transferred to a NC at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10.000 for 1.5 hour at RT. ECL West Pico Plus with Tanon 5200 system.