TNF alpha Rabbit Polyclonal Antibody
Western blot analysis of THP-1, using TNF-α Rabbit pAb at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37℃ for overnight and LPS (1 μg/ml) at 37℃ for 6 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 60s.
| Catalog Number: | AB-90222 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human TNFA. AA range:141-190. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1/500 - 1/2000 Immuohistochemistry(paraffin-embedded tissues) 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000 |
| Molecular Weight: | 16kD |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Form: | Liquid |
| Synonyms: | TNF; TNFA; TNFSF2; Tumor necrosis factor; Cachectin; TNF-alpha; Tumor necrosis factor ligand superfamily member 2; TNF-a |
Applications
Western Blot: 1/500 - 1/2000 Immuohistochemistry(paraffin-embedded tissues) 1:100-300 Immunofluorescence: 1:100-300 ELISA: 1/20000
Immunogen
The antiserum was produced against synthesized peptide derived from human TNFA. AA range:141-190.