TNF alpha
Western blot analysis of extracts of various cell lines, using TNF-α antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL West Pico Plus. Exposure time: 30s.
| Catalog Number: | MAB-94636 |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | TNF-3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human TNF. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:1000 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 |
| Molecular Weight: | 17kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | TNF;DIF;TNF-alpha;TNFA;TNFSF2;TNLG1F;TNF alpha |
Applications
Western Blot: 1:1000 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-233 of human TNF.