Toll Like receptor 4 (S441) (TLR4)

Western blot analysis of extracts of A-549 cells, using TLR4 (S441) antibody at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 5min.

Western blot analysis of extracts of A-549 cells, using TLR4 (S441) antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit Exposure time: 5min.

Catalog Number:AB-83776
Size:100 ug
Concentration:1mg/ml
Host:Rb
Isotype:IgG
Clone:POLY
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 500-600 of human TLR4.
Reactivity:Hu, Ms, Rt
Applications:Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200
Molecular Weight:120kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:TLR4;ARMD10;CD284;TLR-4;TOLL

Applications

Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:100 - 1:200 Immunofluorescence: 1:50 - 1:200

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 500-600 of human TLR4.