Tubulin alpha Mouse Monoclonal Antibody

Western blot analysis of extracts of various cell lines, using Tubulin alpha antibody at 1:1.500 dilution. The Secondary Antibody is HRP Goat anti mouse IgG (H+L).  Lysates / Proteins 25ug/Lane.  
Blocking buffer:  3% nonfat dry milk in TBST.  
Detection:   ECL Fast Pico Kit. 
Exposure time:   5 s

Western blot analysis of extracts of various cell lines, using Tubulin alpha antibody at 1:1.500 dilution. The Secondary Antibody is HRP Goat anti mouse IgG (H+L). Lysates / Proteins 25ug/Lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Fast Pico Kit. Exposure time: 5 s

Catalog Number:MAB-94264
Conjugate:Unconjugated
Size:200 ug
Concentration:1mg/ml
Host:Mouse
Isotype:IgG1
Clone:TU-02
Immunogen:Recombinant protein of human α-Tubulin
Reactivity:Hu, Ms, Rt,
Applications:Western Blot: 1 :500 – 1:5,000 Immunohistochemistry (paraffin embedded tissues): 1:50 – 1:200 Immunofluorescence: 1:20 – 1:100 Immunoprecipitation: 1:20 – 1:50
Molecular Weight:50kDa
Purification:Aff. Pur.
Form:Liquid
Synonyms:TUBA4A;ALS22;H2-ALPHA;TUBA1

Applications

Western Blot: 1 :500 – 1:5,000 Immunohistochemistry (paraffin embedded tissues): 1:50 – 1:200 Immunofluorescence: 1:20 – 1:100 Immunoprecipitation: 1:20 – 1:50

Immunogen

Recombinant protein of human α-Tubulin