Tubulin beta Mouse Monoclonal Antibody
Western blot analysis of extracts of various cell lines, using β-Tubulin antibody HRP conj. at 1:5,000 dilution. Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10,000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Kit. Exposure time: 1s.
| Catalog Number: | MAB-80143 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Ms |
| Isotype: | IgG1 |
| Clone: | TUB2 |
| Immunogen: | Recombinant protein of human β-Tubulin |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:5000 - 1:10000Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200Immunoprecipitation: 1:50- 1:200 |
| Molecular Weight: | 50kDa |
| Purification: | Aff. Pur. |
| Form: | Liquid |
| Synonyms: | M40; TUBB1; TUBB5; CDCBM6; CSCSC1; OK/SW-cl.56 |
Applications
Western Blot: 1:5000 - 1:10000Immunohistochemistry (paraffin-embedded tissues): 1:50 - 1:200Immunofluorescence: 1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200Immunoprecipitation: 1:50- 1:200
Immunogen
Recombinant protein of human β-Tubulin