VEGF-A Rabbit Polyclonal Antibody
Western blot analysis of various lysates, using VEGFA Rabbit pAb at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 30s.
| Catalog Number: | AB-82183 |
| Conjugate: | Unconjugated |
| Size: | 200 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | The antiserum was produced against synthesized peptide derived from human VEGF-A. AA range:110-159 |
| Reactivity: | Human, Mouse, Rat, Pig, Rabbit |
| Applications: | Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300 ELISA:1/10000. |
| Molecular Weight: | 21kD(monomer),42kD(dimer) |
| Purification: | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen |
| Form: | Liquid |
| Synonyms: | VEGFA; VEGF; Vascular endothelial growth factor A; VEGF-A; Vascular permeability factor; VPF |
Applications
Western Blot: 1/500 - 1/2000 Immunofluorescence: 1:50-200 Immunohistochemistry: 1/100 - 1/300 ELISA:1/10000.
Immunogen
The antiserum was produced against synthesized peptide derived from human VEGF-A. AA range:110-159