Vimentin
![Western blot analysis of tissue and cell lysates using chicken pAb to Vimentin, Vim, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. Vim binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, 2C4, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes, and ~70kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.](https://immunologicalsciences.com/wp-content/uploads/2022/01/AB-83918-1.jpg)
Western blot analysis of tissue and cell lysates using chicken pAb to Vimentin, Vim, dilution 1:5,000 in red. [1] protein standard (red), [2] rat whole brain lysate, [3] HeLa, [4] SH-SY5Y, [5] HEK293, and [6] NIH-3T3 cell lysates. Vim binds to the vimentin protein showing a single band at ~50 kDa. The blot was simultaneously probed with mouse mAb to MAP2C/D, 2C4, dilution 1:5,000 in green, revealing multiple bands around 280kDa that correspond to full length MAP2A/2B isotypes, and ~70kDa bands which are MAP2C/D isotypes. MAP2 isotypes are seen only in extracts containing neuronal lineage cells.
| Catalog Number: | AB-83918 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Ch |
| Isotype: | IgY |
| Clone: | POLY |
| Immunogen: | Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli. expressed in and purified from E. coli. |
| Reactivity: | Hu Rt Ms,Cw, Pg, Ho, Dg, Ch, Mk |
| Applications: | Western Blot: 1:5,000. Immunofluorescence: 1:2,500. Immunocytochemistry: 1:2,500 Immunohistochemistry (Frozen): 1:2,500 |
| Molecular Weight: | 50 kDa |
| Purification: | Purified |
| Form: | Liquid |
Applications
Western Blot: 1:5,000. Immunofluorescence: 1:2,500. Immunocytochemistry: 1:2,500 Immunohistochemistry (Frozen): 1:2,500
Immunogen
Full length recombinant human vimentin protein , PROT-r-Vim, expressed in and purified from E. coli. expressed in and purified from E. coli.