Vimentin
Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 90s.
| Catalog Number: | MAB-94625 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | D21H3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Vimentin. |
| Reactivity: | Hu, Ms, Rt |
| Applications: | Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200 |
| Molecular Weight: | 56kDa |
| Purification: | Purified |
| Form: | Liquid |
| Synonyms: | CTRCT30;HEL113;Vimentin;VIM;vimentin |
Applications
Western Blot: 1:500 - 1:2000 Immunohistochemistry: 1:50 - 1:200 Immunofluorescence: 1:50 - 1:200 Immunoprecipitation: 1:50 - 1:200
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human Vimentin.