Vimentin
Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus. Exposure time: 1s.
| Catalog Number: | AB-82376 |
| Conjugate: | Unconjugated |
| Size: | 100 ug |
| Concentration: | 1mg/ml |
| Host: | Rabbit |
| Isotype: | IgG |
| Clone: | POLY |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 367-466 of human Vimentin |
| Reactivity: | Human, Mouse, Rat |
| Applications: | Western Blot: 1:2000 - 1:20000 Immunohistochemistry (paraffin embedded tissues): HC-P 1:100 - 1:500 Immunofluorescence:1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 IP 1:500 - 1:1000 |
| Molecular Weight: | 54kDa |
| Purification: | Affinity purification |
| Form: | Liquid |
| Synonyms: | CTRCT30;HEL113;Vimentin;VIM;vimentin |
Applications
Western Blot: 1:2000 - 1:20000 Immunohistochemistry (paraffin embedded tissues): HC-P 1:100 - 1:500 Immunofluorescence:1:50 - 1:200 Immunocytochemistry: 1:50 - 1:200 IP 1:500 - 1:1000
Immunogen
A synthetic peptide corresponding to a sequence within amino acids 367-466 of human Vimentin