YAP
Western blot analysis of extracts of various cell lines, using YAP1 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti- Rabbit IgG (H+L)at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL West Pico Plus Exposure time: 10s.
| Catalog Number: | MAB-94469 |
| Size: | 100μg |
| Concentration: | 1mg/ml |
| Host: | Rb |
| Isotype: | IgG |
| Clone: | D8H1X |
| Reactivity: | Hu,Ms,Rt |
| Applications: | Western blotting 1:1000 Immunoprecipitation 1:20-1:50 Immunohistochemistry (Paraffin) Unmasking buffer: Immunofluorescence IF Size: 100 ug Concentration: 1mg/ml Clone: D8H1X 1:50-1:200 1:50-1:200 |
| Molecular Weight: | 65-75 kDa |
| Purification: | Monoclonal antibody is produced by immunizing animals with recombinant protein specific to the carboxy terminus of human YAP protein. The epitope corresponds to a region surrounding Pro435 of human YAP isoform 1. This sequence region is 100% conserved among all known isoforms of human YAP protein. |
| Form: | Liquid |
Applications
Western blotting 1:1000 Immunoprecipitation 1:20-1:50 Immunohistochemistry (Paraffin) Unmasking buffer: Immunofluorescence IF Size: 100 ug Concentration: 1mg/ml Clone: D8H1X 1:50-1:200 1:50-1:200